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Seed-Based Restoration

Seeds are the foundation of ecosystem restoration. The 2023 Native Plant Material Survey (in process) was an opportunity to articulate the collective need for access to more native seeds in order to reach restoration goals and expand restoration capacity. Discussions around "Disturbance-Ready Restoration" at the 2023 Pacific Entomological and Botanical (PEB) meeting indicated that seeds are key resources to respond to Extreme Climate Events (e.g., post-fire, post-wind storm), and limitations in the availability of seed quantity and quality restrict managers ability to respond to ECEs. In a subsequent set of conversations at the 2024 Hawaiʻi Conservation Conference (HCC) during a workshop on "Climate-Change Resilient Restoration", seed availability was articulated as a barrier to achieving restoration goals within several ecosystem contexts. Furthermore, knowledge of seed based restoration projects and efficacy in Hawaiʻi remains largely unknown. At the 2024 Hawaiʻi Island Restoration Forum, three seed broadcasting projects were highlighted. These presentations address the logistics of conducting a seed broadcast including collection effort, seed cleaning, seed deployment. Monitoring is a key component of these projects however, monitoring strategies and any results are not a part of this resource.

 

Click on any of the thumbnails below to peruse the presentation as a pdf. Use the menu below the presentations to compare details across projects.

Hawaiʻi Volacanos National Park
(HVNP)
Waikōloa Dry Forest Initiative
(WDFI)
Nāpuʻu Natural Resources Management
(NNRM)
KeauhouFire_1.png
KeauhouFire_2.png
WaikoloaFire.png
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Hand Broadcast
Aerial Broadcast
Sections
Sections
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Site Details

HVNP
WDFI
NNRM
August 2022
Leilani Fire
August 2018
Waikōloa Fire
August 2018
Keauhou Fire

Fire name

Month Year (of fire)

Total fire size

3,739 acres burned total 2,978 acres within park
18,000 acres burned total 
21.5 acres within Preserve
15,000 acres total
10,000 acres within
management area

Landcover traits

Cenchrus setaceum, (Fountain grass)
non-native grassland
Māmane shrubland and
koa mesic forest 
ʻŌhiʻa-dominated scrubland
(~2500 acres) and C. setaceum 
non-native grassland

Elevation (ft)

*3,840-5,740'
**900-1100'
*3,400–5,500'

Mean annual rainfall (in)

40-60"
9-12"
10–29"

Management overview

  • Fencing in place before fire 
  • Seed broadcasting started immediately following fire
  • Fencing in place before fire
  • Conducted grass-control before broadcast
  • Fenced 18 acres before broadcast, within 240 unfenced management area 
  • Conducted grass-control before broadcast
  • Also conducted archeological surveys and installed predator control (A24s)
*Elevation range of fire
**Elevation range of entire preserve
Broadcast Details

Broadcast Details

HVNP
WDFI
NNRM

Method

Hand broadcast

  • Larger seeds like wiliwili and ʻāwikiwiki had a hard time finding their way into the soil crevices of the pāhoehoe

  • Conducted aerial and ground surveys to delineate post-fire restoration area​

  • remote access, ~1.5 hr drive

  • ATV seed hopper modification

  • Calibration with similar sized bird seed

  • lightly scratch soil surface with rakes

  • deposit seeds, lightly cover with mulch/soil

  • in shrubland: surface sow onto lava rock, ʻiliahi hand push into soil (to discourage rat predation)

  • For māmane, broadcasted untreated and acid-treated seeds to facilitate germination waves

Hand broadcast

Aerial Broadcast

Design

  • 34 transects spaced 200 meters apart

  • 10 m diameter circular plots at 50 meters apart

  • plot center marked and meter tape laid out

  • plots rejected if over 50% invasive grass or if too dangerous

  • recorded grass/fern cover and burn severity

  • Gridded out burn area into .5 acres (squares where possible)

  • Total of 43 grids

  • 1 bag of seed and 1 bucket of ʻaʻaliʻi per grid

  • 4.5 acre plots

  • four treatments and control

    • 6,000 lb mulch/acre plus seed​

    • 3,000 lb mulch/acre plus seed

    • seed only

    • control

  • two-1 acre calibration plots

Broadcast Totals (total weight, est. # total seeds, total area)

  • Nearly 2 million seeds total: 792,506 into Koa Forest, 564,000 into Māmane Shrubland

 

 

  • plots encompassed 1,146 acres total (across 3,000 acres)

  • 25182 g of seed total (55.5 lbs)​

  • ​+3.6 million ʻaʻaliʻi seeds

  • +10.6 million seeds ​of ʻāweoweo, ʻilima and wiliwili combined 

  • 21.5 acres total

  • 172 lbs of seed total (78017.8 g)

  • 116.75 lbs of seed = +22M (ʻaʻaliʻi, ʻāweoweo, ʻilima)

  • ~18 acres

Broadcast effort

230 workerdays

900-1100'

one 8 hr flight day and  one 4 hr flight day

Monitoring

Yes--preliminary results presented as poster during 2024 HCC

Yes--results published as Masters Thesis (see Warner 2023)

Yes--project underway

Notes

  • ​Covering such a large area before grass could recover was not possible, by second year grass recovery was already 100% 

  • December 2020 Kīlauea Eruption added layer of complexity and hardship for seedlings

  • Seedling recruitment surveys difficult to conduct after invasive grass recovery

  • Conducted two post-broadcast grass-specific herbicide treatments: 1) three treatments over 18 months (i.e., short term) and 2) bi-annual treatments for three years (i.e., long term)

  • The long-term treatment significantly increased seedling recruitment and survival (see Warner 2022 for more information)

  • Seed calibrating and trouble shooting

  • Initially intended to use silica sand with seed to spread seeds in the large hopper

  • Hopper was too large for the job

  • Calibration had us going back to the drawing board

Seed Collection

Seed collect effort (amount of seed/ number of person hours) is variable. Species vary in the number of seeds per fruit. Abundance and density varies across the landscape, shaped by habitat quality and degradation, influencing both the number of individuals collected from (i.e. founders) and collection range. Fruit availability varies, both within a site and from year to year. Collection efforts impacted as both individuals and different species seeding at the same time. Annual variation in seed availability can occur in response to environmental conditions, predation, and their interaction, further generating variation in seed collection efforts for any given species. As such, seed collection efficiency will vary depending on site and plant density.

 

An example of this within species variation is seen with NNRM collecting 5 contractor bags of ʻaʻaliʻi with 4 people in 4 hours at one site, but 1 contractor bag with 4 people in 5 hours at another site. A summary of total weight, number of founders and collection ranges for 12 species is described in the table below.

Total number of seeds

ʻaʻaliʻi: 3,604,700 (clean) || 12430000 (110:175)

​ʻāweoweo: 10850000 || 9275000 (5.3:175)

ʻilima: 105000 || 304500 (1.45:175)

PC: NNRM

Contractor bags full of native seeds.

HVNP has been conducting park-wide seed collection since the 1990's, with seed collection intensifying in early 2000’s following Pepeiao fire in ʻōhiʻa woodland. Most seed for this project was collected within last 5-10 years–for details on collection efforts. Technical reports describing post-fire restoration projects in HVNP provide insight into collection effort and broadcast totals. See Duffy et al. (2007) and McDaniels et al. (2008), full citations provided in Additional Resources. Regardless, the project still felt limited by the quantity of seed available, especially in terms of species diversity. For Koa Forest they broadcasted ʻaʻaliʻi, pilo, māmane, naio and ʻiliahi. In the Māmane Shrubland, they broadcasted māmane, koʻokoʻolau and pua kala. Collections originated from Kīlauea and Mauna Kea volcano, within the park, excluding the Kahuku Unit. Founders varied from 10’s for pua kala to 100’s for māmane. Future efforts would ideally include a wider range of species, especially native grasses, sedge, wood rush species to fill ground cover niche, as well as other common and vigorous species such as ʻakala, mamaki and hōʻawa.

 

For the leeward dry forest restoration projects, partnerships like the Dry Forest Hui were crucial for increasing seed access and greatly widened the collection range, the number of individuals and ultimately the amount of seed gathered. In total, NNRM collect 98 lbs of seed over 456.5 worker hours, and estimated that 1 lb of seed took 4.65 hours to collect. WDFI found that volunteers love collecting seeds, with seed collection and storage persisting as a crucial part of their summer programming.

Collection Traits for 12 dry to mesic forest species by two Dry forest management programs

Species

Total Weight

Number of Founders

Collection Range

(grams / pounds)

ʻaʻaliʻi 

Dodonea viscosa

WDFI: 14450 / 31.9--with fruit

            1417 / 3.1--cleaned 

NNRM: 43091.3 / 95 

             6803.9 / 15 donated

WDFI: 100s

NNRM:900-1100'

WDFI: 50 acres at WDF, 25 acres               at Puʻu Waʻawaʻa

NNRM: PTA and/or Puʻu Waʻawaʻa

ʻāweoweo (1085000)

Chenopodium oahuense

WDFI: 2807 / 6.2

 

NNRM: 226.8 / 0.5

              2268 / 4.8 donated

WDFI: 25+

 

NNRM: 900-1100'

WDFI: 50 acres at WDF, 25 acres               at Puʻu Waʻawaʻa

NNRM: PTA and/or Puʻu Waʻawaʻa

ʻāwikiwiki

Canavalia hawaiiensis

WDFI: 3884 / 8.6 donated

WDFI: unknown (donated)

WDFI: unknown (donated)

ʻilima 

Sida fallax ​​

WDFI: 210 / 0.5

NNRM: 159 / 0.35 

              499 / 1.1 donated

WDFI: 100s

NNRM:900-1100' 

WDFI: 10 acres at WDF

NNRM: PTA and/or Puʻu Waʻawaʻa

kāwelu

Eragrostis sp

NNRM: 4.5 / 0.01

NNRM:900-1100' 

NNRM: PTA and/or Puʻu Waʻawaʻa

kōlea

Myrsine spp  

NNRM: 114 / 0.25

NNRM:900-1100' 

NNRM: PTA and/or Puʻu Waʻawaʻa

kolomona

Senna gaudichaudiana

WDFI: 887 / 1.96

WDFI: 10+

WDFI: 50 acres at WDF

koʻokoʻolau

Bidens spp  

WDFI: 28 / 0.06

WDFI: 25+

WDFI:  25 acres at Puʻu Waʻawaʻa

māmane

Sophora chrysophylla 

WDFI: 510 / 1.1 donated

NNRM: 1133.9 / 2.5 

             24040.4 / 53 donated

WDFI: unknown (donated)

NNRM: 900-1100'

WDFI: unknown (donated)

NNRM: PTA and/or Puʻu Waʻawaʻa

pua kala, puakala

Argemone glauca

WDFI: 164 / 0.36

NNRM: 0

              136.1 / 0.3  donated

WDFI: 25+

NNRM: unknown (donated)

WDFI: 10 acres at WDF

NNRM: unknown (donated)

ʻūlei

Osteomeles anthyllidifolia

WDFI: 187 / 0.41 (donated)

WDFI: unknown (donated)

WDFI: unknown (donated)

wiliwili 

Erythrina sandwicensis 

WDFI: 638 / 1.41

WDFI: 25+

WDFI:  275 acres at WDF

Seed Processing

Cleaning method and overall processing effort is also dependent on species. For example, it can take days to clean māmane collections but up but minutes for puakala and koʻokoʻolau. HVNP found that the Dybvig Seed Cleaner + aspirator works great for processing large amount of ʻaʻaliʻi but less effective for māmane. However, this tool also has potential for soft-fruit seeds, such naio, ʻiliahi & pilo. WDFI found professional seed cleaning costs to be prohibitive, although this may vary depending on the size of your project. They promoted awareness for the potential contaminants that may arise with DIY seed cleaning, such as microplastics and excess fruit material. NNRM highlighted the high time input in processing (as well as collecting) seed, emphasizing the value in having the correct equipment and tools to process seeds most efficiently. For example, NNRM estimated that it took ~35 worker hours to process 98 lbs of ʻaʻaliʻi seed, translating to ~35 min to clean 1 lb of seed. The program evaluated their seed material development effort to be ~4 hrs to collect and process 1 lb of seed, valuing that seed at ~$140/lb. In total, the 98 lbs of seed they collected and processed cots $13,720. WDFI provides a cost-effort summary for four species in the table below.

Seed Cleaning Methods

Cleaning methods include:

  • Rubber mallet and pillowcase (māmane)

  • Hand roll and winnow (ʻaʻaliʻi)

  • Food processor (fleshy fruit--pilo, naio & ʻiliahi) 

  • Hand clean (koʻokoʻolau & puakala) 

  • Aspirators​ (Pictures 1 and 2)

  • Weedwhacker + bucket (ʻaʻaliʻi)–Picture 3

Picture 1

Home made aspirator, custom design WDFI 

Picture 2

PC: NNRM

Seed Column Blower

Picture 3

PC: NNRM

Weed whacker +

Bucket (on wheels)

WDFI Seed Effort Table: Assessing species-specific cost-effort for developing seed broadcast materials

Species

*Seeds/ lb
**Effort/ lb
***Cost/ lb

ʻaʻaliʻi

Dodonea viscosa

113K

10-20 hrs

$300-600

ʻāweoweo

Chenopodium oahuense

1.75M

4-6 hrs

$120-360

ʻilima

Sida fallax ​​

210K

30 hrs

$900

wiliwili

Erythrina sandwicensis 

225

3-4 hrs

$90-120

*number of seeds per pound, average seed weight data from Lyon Arboretum
** time to collect and process one pound of seed
***rated at $30/hr
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Takeaways

HVNP found ʻaʻaliʻi to regenerate readily on its own post-fire, leading to a shift in species-selection to focus on species that have lower potential for natural recovery post-fire. Recovery appears to vary given pre-fire vegetation, with different restoration strategies appropriate for bunch grass (Broomsedge, Andropgon sp.) vs. mat grass (Kikuyu, Cenchrus clandestinus). Furthermore, they expressed a desire to target areas with high burn severity.

 

Over their monitoring period, WDFI has seen that long-term weed management has a direct correlation with increased number of stems, plant vigor, and overall plant height for native species. At the time of this presentation, Koʻokoʻolau was on its 4th or 5th generation, ʻāweoweo was continuing to seed with new plants beginning to germinate, and ʻaʻaliʻi fruit was becoming a visible component of the leaf litter/ soil.
 

NNRM highlighted the need to anticipate catastrophic events (fires) and value of prepping ahead of time. Projects require flexibility and being able to adapt when changes come up is key. When working in remote sites, small problems can easily become big ones. Budgeting time (and finances) with these considerations in mind with behove you and the success of your project.

All the programs found that the amount of seeds that were in storage were not enough to address their post-fire restoration needs and emphasized the need for more seed collection of a wider diversity of species to be able to conduct effective and timely post-fire restoration. HVNP, relying heavily on ~15 years of seed collection broadcast around 2 million seeds in over 1,000 acres. In contrast, WDFI broadcast over 3.6 million ʻaʻaliʻi seeds alone across their 21.5 acre restoration site and NNRM broadcast 172 lbs of seed by helicopter--with ~116 lbs of those seeds consisting of over 22 million seeds.

 

While both WDFI and NNRM benefited from historic collection efforts, they keenly demonstrate the effort required to prepare for large-scale, seed-based restoration. In particular, they highlight a need to shift from opportunistic collections to dedicated teams with focused collection targets to overcome collection challenges such as inter-annual variation and founder limitations and ultimately to acquire have the right seed at the right time, and from the right place.

 

There are still outstanding storage limitations, including unknown viability of seeds in storage without conducting time-intensive germination/viability trials and certain species do not store well, both leading to an ongoing need to recollect to keep enough viable seed on hand. Lack of germination trials also leads to questions of how much of seed broadcasted are actually viable. 

Questions surrounding founder diversity remain, especially when a project has access to only a few wild individuals. In a later part of the forum, groups discussed potential frameworks for evaluating seed zones for a given species. Some key questions include origin (indigenous vs endemic), dispersal mechanism, and historic ranges of bird dispersers.


As the capacity to conduct seed-based restoration continues to grow, the question remains–What do we charge for a pound of seeds? Given current tools, a single pound of seed may take upwards of 50 hours to collect and process. As demonstrated throughout these presentations seed collection and processing are both time consuming. Place-based knowledge is central to seed collection, a knowledge base that takes time and practice to develop and as such, seed collectors are specialists. As collection efforts increase, there is a need for more efficient tools to process large seed batches.

Seed-based restoration goes hand-in hand with an increase in the collection and storage of common seeds. Ungulate exclusion is going to be necessary for most species. Weed management can have a positive impact on restoration success. 

Additional Resources

The information above largely focused on the logistics of conducting seed-based restoration. A survey and synthesis of both grey and journal published papers of research describing the efficacy of seed broadcasting in Hawaiʻi--especially in dry forest ecosystems--is still necessary. The following citations were referenced in the text above and provide additional insight into collection and cleaning efforts, monitoring schemes and results. 

Duffy, D. C., Loh, R., McDaniel, S., Schultz, M., Ainsworth, A., Benitez, D., Palumbo, D., Smith, K., Tunison, T., Vaidya, M., & Smith, K. (2007). Rehabilitation of seasonally dry ʻōhia woodlands and mesic koa forest following the Broomsedge Fire, Hawaiʻi Volcanoes National Park. PCSU Technical Report, 147. 21pp.

McDaniel S, Loh R, Dale S, Smith K, Vaidya M. 2008. Rehabilitation of ʻōhiʻa-swordfern (Metrosideros polymorpha-Nephrolepis multiflora) woodlands following the Kupukupu Fire, Hawaiʻi Volcanoes National Park. PCSU Technical Report, 160. 24pp.

 

Warner, T. (2023). Success of post-fire broadcast seeding as a tool for restoration of a Hawaiʻi dry forest. University of Hawaiʻi, Mānoa. Thesis. 118pp.

Explore Hawaiʻi-centered Seed resources through Laukahi: The Plant Conservation Network and the facilitator of the Hawaiʻi Seed Bank Partnership, including the Hawaiʻi Seed Bank User's Guide

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